Analysis Note

ControlHuman brain tissue and extracts from mouse brain and rat brain tissue.

Application

This Anti-Glutamate Receptor 2 & 3 Antibody is validated for use in IH(P), IC, IH, IP, WB for the detection of Glutamate Receptor 2 & 3.

Immunohistochemistry:
A previous lot was used on paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Final concentrations used were 1-3 µg/mL.

Immunoprecipitation:
When linked to immobilized Protein A, a previous lot of this antibody immunoprecipitated detergent-solubilized receptor from brain or transfected cells.

Research CategoryNeuroscience

Research Sub CategoryNeurotransmitters & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcategorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition.

Immunogen

Carboxy terminus peptide of rat GluR2 conjugated to BSA with glutaraldehyde (EGYNVYGIESVKI).

Epitope: C-terminus

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified rabbit polyclonal serum lyophilized from buffer containing 0.01M phosphate, 0.09M NaCl, pH 7.2. 1% BSA and 0.1% sodium azide.

ImmunoAffinity Purified

Quality

Evaluated by Western Blot on rat brain lysate.

Western Blot Analysis:
1:1000 dilution of this antibody detected glutamate receptor 2 & 3 on 10 µg of rat brain lysate.

Specificity

Expected to cross-react with human, rat, monkey, gerbil, guinea pig, and hamster.

It recognizes both GluR2 and GluR3 which have nearly identical carboxy terminal sequences. Western blot analysis of rat brain shows two poorly separated bands which comigrate with GluR2 and GluR3 expressed in transfected cells. GluR2 migrates slightly ahead of GluR3. No cross-reaction with GluR1 or GluR4. The antibody appears to recognize GluR 2 & 3 in several other species, but has not been extensively characterized in these cases.

Storage and Stability

Maintain lyophilized material at +2-8°C or -20°C for up to 6 months after date of receipt.
Reconstitute to 500 µL with sterile distilled water. Store reconstituted material frozen (-20°C or -80°C) in undiluted aliquots for up to six months. Diluted antibody containing 0.1% sodium azide for Western blot analysis can be stored for several weeks at +2-8°C and reused repeatedly.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

108 kDa